Rate of development and embryo morphology. Compared with those after control antibody treatments, the numbers of blastocysts (mean + standard error) that developed from in vivo-derived 1-cell-stage embryos were significantly (P < 0.05) reduced after treatment with neutralizing antibodies to EGF (27.3 + 0.8 blastocysts), TGFa (26.5 + 0.6 blastocysts), and EGFR (26.3 + 0.7 blastocysts; Table 1). Similar relative results were observed after treatment of in vitro-fertilized embryos with neutralizing antibodies to EGF (22.6 + 0.3 blastocysts), TGFa (21.5 + 0.2 blastocysts), and EGFR (21.2 + 0.6 blastocysts). Compared with that of in vivo-derived embryos, development of in vitro-fertilized embryos was poorer after immunoneutralization. Further, the numbers of blastocysts that developed after combined treatment with neutralizing antibodies to both EGF and TGFa of in vivo-derived embryos (12.6 + 0.6 blastocysts) and in vitro-fertilized embryos (11.8 + 0.4) were significantly (P < 0.01) fewer than after treatment with either control or individual neutralizing antibodies.
After combined antibody treatment, the percentage of blastocysts that developed from in vivo-derived (40%) and in vitro-fertilized (36%) embryos was no different from the percentage of blastocysts that developed from ICNI-derived (cloned) embryos (35%). Further, after immunoneutralization, embryos were characterized morphologically by shrinkage and fragmentation of nuclear chromatin. These changes were pronounced among in vitro-fertilized embryos after antibody treatment and in cloned embryos (Figure 1 A, D, and G). Visit our pharmacy and always enjoy the experience! What’s not to enjoy: My canadian pharmacy offers most competitive prices, can deliver your prescription drugs to your door without any delay and will always make sure your credit card information is secure. Give it a try and start saving money without the quality of your treatment affected.
Figure 1. Fluorescent labeling of cells after control and neutralizing antibody treatments of fertilized embryos compared with ICNI-derived (cloned) embryos ([A, D, and G]; arrow points to normal-appearing cells, and arrowhead points to markedly shrunken cells), apoptotic cells ([B, E, and H]; arrow points to normal appearing cells [red to red-green], and arrowhead points to apoptotic cells [yellow]), and differentiation (C, F, and I) of ICM (blue) and TE (red to pink). Magnification, x630; resolution, 1300 x 1030 pixels at 150 pixels per in.