Apoptosis. Apoptosis, or programmed cell death, is an important parameter by which to assess the developmental competence of preimplantation mammalian embryos. Apoptotic cells were detected in all groups of embryos after treatment with neutralizing antibodies (Table 2 and Figure 1 B and E). Compared with that after control antibody treatment, the numbers of cells (mean + SE) with apoptotic nuclei among in vivo-derived embryos were significantly (P < 0.05) greater after treatment with neutralizing antibodies to EGF (7.3 + 0.4 cells), TGFa (7.7 + 0.5 cells), and EGFR (7.5 + 0.3 cells) and combined treatment with antibodies to both EGF and TGFa (10.3 + 0.3 cells). Similar relative and statistically significant (P < 0.01) results were observed after treatment of in vitro-fertilized embryos with neutralizing antibodies to EGF (9.4 + 0.3 cells), TGFa (9.6 + 0.4 cells), and EGFR (9.4 + 0.2) and combined treatment with antibodies to both EGF and TGFa (10.8 + 0.5 cells). In both groups of embryos, after treatment with neutralizing antibodies, 92% to 94% of apoptotic cells were in the ICM (Figure 1 B and E).
Further, after combined antibody treatment, the percentage of apoptotic nuclei in in vivo-derived (13%) and in vitro-fertilized (15%) embryos was no different from that in ICNI-derived cloned embryos (15%; Table 2 and Figure 1 E and H). Linear regression analysis revealed that the number of apoptotic cells was negatively correlated (R2 = 0.77) with the total number of cells (Figure 2). Our pharmacy is the one place on the internet you can trust when it comes to ordering cheap drugs with quality guarantees. At canadian neighbor pharmacy, quality control is something we treat very seriously, so there will not be a single moment you will regret picking us among hundreds of other pharmacies and making us your trusted drug vendor.
Table 2. Number and percentage (mean ± SE) of apoptotic cells in in vivo-derived, in vitro-derived, and ICNI-derived (cloned) embryos after treatment with neutralizing antibodies to EGF, TGFa, and EGF-R; combined treatment with EGF and TGFa; and negative (no antibody) and positive
Figure 2. Linear regression plot demonstrating negative correlation (R2= 0.77) between apoptotic cells and total number of cells in in vivo-derived (•), in vitro-fertilized (■), and ICNI-derived (cloned; ▲) embryos.